EFEK KOMBINASI ASAM VALPROAT DAN NANO KITOSAN KUMBANG TANDUK (Xylotrupesgideon) TERHADAP VIABILITAS DAN SITOTOKSISITAS SEL KANKERLIDAH (HSC-3)

Komariah Komariah, Abdining Ageng, Indra Kusuma

Abstract


Kanker lidah merupakan salah satu kanker terbanyak dengan mortalitas tertinggi di dunia. Radioterapi dan kemoterapi pada kanker relatif terbatas karena toksisitasnya yang tinggi dan efek samping yang ditimbulkan. Pengembangan asam valproat dan nano kitosan Xylotrupes gideon merupakan strategi baru untuk terapi antikanker berbahan alami yang diharapkan meminimalkan efek samping terapi standar yang ada. Penelitian ini bertujuan untuk mengetahui pengaruh paparan kombinasi asam valproat dan nano kitosan yang berasal dari X. gideon terhadap viabilitas dan sitotoksisitassel HSC-3. Penelitian menggunakan kultur sel HSC-3 yang terbagi dalam empat kelompok perlakuan, yaitu dengan pemberian kombinasi asam valproat dan nano kitosan dengan perbandingan 1:1, 1:2, 1:3, dan tanpa paparan kombinasi asam valproat dan nano kitosan (kontrol). Penelitian dilakukan untuk melihat viabilitas dan sitotoksisitassel HSC-3 menggunakan microplate reader pada panjang gelombang 450nm. Hasil uji viabilitas dan sitotoksisitas paparan kombinasi memperlihatkan perbedaan signifikan dibandingkan dengan tanpa paparan kombinasi asam valproat dan nano kitosan  (p<0.05). Hasil penelitian memperlihatkan semakin tinggi paparan kombinasi asam valproat dan nano kitosan memperlihatkan penurunan  viabilitas serta peningatan sitotoksisitas sel HSC-3.


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References


Arrouze F, Essahli M, Rhazi M, Desbrieres A, Tolaimate, 2017, Chitin And Chitosan: Study of the Possibilities of Their Production by Valorization of The Waste Of Crustaceans And Cephalopods Rejected In Essaouira. Journal of Materials and Environmental Sciences. Vol 8 no 7, 2251-2258.

Azuma K, Osaki T, Minami S, Okamoto Y, 2017, Anticancer and Anti-inflammatory Properties of Chitin and Chitosan Oligosaccharides. Journal of Biomaterials, vol 6, 33-34.

Babu A, Ramesh R, 2017, Multifaceted Applications of Chitosan in Cancer Drug Delivery and Therapy. Marine Drugs, vol 15 no 4, 96.

Eckschlager t, Plch j, Stiborova M, Hrabeta J, 2017, Histone Deacetylase Inhibitors as Anticancer Drugs, International Journal of Molecular Sciences, vol 18 no 1414, 1-25.

Gaddikeri, Kavitha, Deepak D. Bhorgonde, 2016, Oral Squamous Cell Carcinoma Of Tongue In Young Patient – A Rare Case Report And Review Of Literature. annals of Int Med and Dent Res. Vol 3 no 1, 1-6.

Ganzhu F, 2013, Enhanced Immune Response And Protective Effects Of Nano-Chitosan-Cased DNA Vaccine Encoding T Cell Epitopes Of Esat-6 And FL Against Mycobacterium Tuberculosis Infection. PLos ONE, vol 8 no 4, 1-7.

Gurvich, Nadia, Oxana M, Meinkoth J, Klein P, 2004, Histone Deactylase Is A Target Of Valproic Acid-Mediated Cellular Differentiation. Cancer Research.Vol 64 no 3,1079-86.

International Agency for Research on Cancer. Globocan 2012: Estimated Cancer Incidence, Mortality And Prevelance Worldwide In 2012. France: International Agency for Research on Cancer.

Khandekar S, Badgey P, Tiwari R, 2006, Oral Cancer And Some Epidemiological Factors: A Hospital Based Study. Indian J Community. Vol 31 no 3, 157-159.

Komariah, Luki A, 2012, Preparasi Dan Karakterisasi Kitin Yang Terkandung Dalam Eksoskeleton Kumbang Tanduk Rhinoceros Beetle (Xylotrupes gideon L) dan Kutu Beras (Sitophilus oryzae L). Seminar Nasional IX Pendidikan Biologi FKIP UNS, 648-654

Komariah, 2013, Karakterisasi Kitin Dan Kitosan Yang Terkandung Dalam Eksoskeleton Kutu Beras (Sitophilus oryzae). Prosiding Seminar Nasional Pendidikan Biologi FKIP UNS, 144-153.

Komariah, Kiranadi B, Winanto A, Manalu W, Handharyani E, 2017, Pemberian asam valproat pada induk tikus bunting menghambat sintesis insulin pada sel otak anak tikus. Majalah Kedokteran Bandung, vol 49 no.3, 124-132.

Komariah, Manalu W, Kiranadi B, Winarto A, Handharyani E, Roeslan M.O, 2018, Valproic Acid Exposure Of Pregnant Rats During Organogenesis Disturbs Pancreas Development In Insulin Synthesis And Secretion Of The Offspring.Toxicological Res, vol 2, 173-182.

Kumar R, Rajkhowa G, Dhar N, Rajan R, 2011, A new Record Of Xylotrupes gideon (Linnaeus) (Coleoptera: Scarabaeidae) On Persia Bombycina, Kost. From India. Munis Entomology & Zoology, vol 6 no 1, 173-175.

Liu, Shaofang, Jie Sun, Lina Yu, Chushu Zhang, Jie Bi, Feng Zhu, Mingjing Qu, Chen Jiang, 2012, Extraction And Characterization Of Chitin From The Beetle Holotrichia Parallela Motschulsky.Molecules. Vol 17 no 4, 4604-4611.

Luca M, Cleris L, Magistroni V, Piazza R, Boschelli F, Formelli F, Passerini CG, 2009, Valproic Acid Enhances Bosutinib Cytotoxicity In Colon Cancer Cells,Int. J. Cancer, vol 124, 1990–1996.

Naji T, Zakeri Ghaderi, Erfani M, 2016, The Effects of Valproic Acid on Viability of MCF-7 Cell Line, Proceedings of 2016 International Conference on Cellular & Molecular Biology and Medical Sciences, vol 21 no 22, 1-7.

Ohnishi, Shunsuke, Takeda S, 2015, Herbal Medicines For The Treatment Of Cancer Chemotherapy-Induced Side Effects.Frontiers in Pharmacology, vol 6, 6:14.

Pracaya, 2008, Hama dan penyakit tanaman. Penebar Swadaya. Jakarta

Ribeiro I, Rodrigues J, 2017, Cytogenetic, Genomic, And Epigenetic Characterization Of The HSC-3 Tongue Cell Line With Lymph Node Metastasis. Journal of Oral Science. Vol 1, 70-81.

Rivera C. 2015, Essentials Of Oral Cancer. Int J Clin Exp Pathol,vol 8 no 9, 11884-94.

Sang Z, Sun Y, Ruan H, Cheng Y, Ding X, Yu Y, 2016, Anticancer Effects Of Valproic Acid On Oral Squamous Cell Carcinoma Via Sumoylation In Vivo And In Vitro. Experimental And Therapeutic Med. Vol 2, 3979-3987.

Sebastian C, 2010, Molecular And Therapeutic Potential And Toxicity Of Valproic Acid. Journal Of Biomed And Biotech, 1-18.

Sharma P, Saxena S, Aggarwal P, 2010, Trends In The Epidemiology Of Oral Squamous Cell Carcinoma In Western UP: An Institutional Study. Ind J Dent Res, vol 21,316-19.

Sianipar, Fronika N, Purnamaningsih R, Rosiana R, 2016, Pengembangan Tanaman Keladi Tikus ( Typhonium Flagelliforme Lodd.) asal indonesia sebagai obat antikanker. ETHOS.Jurnal Penelitian dan Pengabdian. 65-74.

Zhao L, Lu-E Shi , Zhi-Liang Zhang , Jian-Min Chen , Dong-Dong Shi , Jie Yang, Zhen-Xing Tang, 2011, Preparation And Application Of Chitosan Nanoparticles And Nanofibers. Brazilian Journal of Chemical Engineering. vol 28 no 3, 353-362.


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