DIAGRAM FILOGENIK HASIL SEKUENS BASA DNA MENGGUNAKAN PROGRAM MEGA-7 (MOLECULAR EVOLUTIONARY GENETICS ANALYSIS)

Harumi Yuniarti, Bambang Cholis S, Astri Rinanti

Abstract


Sequencing techniques Deoxyribonucleic Acid (DNA) at this time is a method to identify the genetic basis of living
beings, including to determine the accurate genetic relationship of several DNA sequence data. Kinship clearly and
easily can be expressed in the form of a phylogenetic tree diagram (Phylogenetic Tree). This diagram represents
kinship obtained from the elektroferogram process of electrophoresis sequencing of DNA bases. From some sequences
experimental results using sequence analysis application program gradually, starting from installing, editing, and
alignment so as to obtain the analysis and phylogenetic branching diagram view. This study uses a sequence analysis
program Mega-7 (Molecular Evolutionary Genetics Analysis) to obtain a phylogenetic diagram for high accuracy
and relatively easy to operate. This program can translate image data into nucleotide bases that shows the kinship of
the distance the nearest branch with a standard sequence. The major advantage of this program is that it can do some
analysis in a single program.

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DOI: http://dx.doi.org/10.25105/pdk.v1i2.1457

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