Efek Ekstrak Daun Pluchea indica terhadap Hambatan Pertumbuhan Porphyromonas gingivalis

Taufiq Ariwibowo, Meiny Faudah Amin, Putri Nur Pratiwi

Abstract


Background: Porphyromonas gingivalis is one of the bacteria causes primary root canal infections. Bacterial elimination using root canal irrigation agents is important in successful root canal treatment. NaOCl as the gold standard is known to have disadvantages such as irritating periapical tissue. Active compounds in Pluchea indicaleaves such as flavonoids, tannins and terpenoids posses antibacterial effects. Objectives: To determine the effect of Pluchea indica leaves extract on P. gingivalis growth inhibition. Method: This study is an in vitro experimental laboratory of  agar well difusion method used Pluchea indica leaves extract in several concentrations (6,25%, 12,5%, 25%, 50%, and 100%) and NaOCl 5,25% as a positive control. The sample in this study was P. gingivalisResult: This study showed that the diameter of inhibitor zone against P. gingivalis was exists on concentration of 50% and 100%, their values are 10,216 mm and 13,023 mm. The positive control showed an average inhibition zone of 12.803mm. Conclusion: Pluchea indica leaves extract showed antibacterial effect against P.gingivalis at the concentration of 50% and 100%. The 100% concentration had the largest inhibition zone and was equivalent to positive control in inhibiting P.gingivalis.


Keywords


Inhibitory zone; Pluchea indica; Porphyromonas gingivalis

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References


Singh H. Microbiology of endodontic infections. (2016) Journal of Dental Oral Health, Vol.2, No.5, pp.1–4.

Peciuliene V, Maneliene R, Balcikonyte E, Drukteinis S. (2008) Microorganisms in root canal infections : a review, Baltic Dental Maxillofacial Journal, Vol.10, No.1, pp.4–9.

Sedgley C. (2017). Virulence of endodontic bacterial pathogens. In: Fouad AF, editor. Endodontic microbiology. 2nd ed. Chapel Hill: John Wiley & Sons, Inc. pp.158-169.

Olsen I, Dahle G. (2004) Salient virulence factors in anaerobic bacteria, with emphasis on their importance in endodontic infections, Endodontic Topic, Vol.9, pp.15–26.

Machado Silveira L, Silveira C, Martos J, Suita de Castro L. (2013) Evaluation of the different irrigation regimens with sodium hypochlorite and EDTA in removing the smear layer during root canal preparation, Journal of Microscopy and Ultrastructure, Vol.1, No.1, pp. 51–56.

Fatimatuzzahra N, Rahayu F, Ningsih NS, Feny, Darsono A, Salasia SIO. (2016). Efek antikariogenik ekstrak daun beluntas (pluchea indica) sebagai penghambat pertumbuhan streptococcus mutans penyebab karies gigi, Jurnal sain Veteriner, Vol.34, No.2. pp.182–187.

Syafira AF, Masyhudi, Yani S. (2019). Efektivitas ekstrak etanol daun beluntas pluchea indica less terhadap bakteri saliva secara in vitro, ODONTO Dental Journal, Vol.6, No.2, pp.68–75.

Pargaputri AF, Munadziroh E, Indrawati R. (2016). Antibacterial effects of pluchea indica less leaf extract on e.faecalis and fusobacterium nucleatum (in vitro), Denta Jurnal Kedokteran gigi, Vol.93, No.56, pp.93–98.

Nahak MM. (2013). Ekstrak etanol daun beluntas (Pluchea indica.L.) dapat menghambat pertumbuhan bakteri Streptococcus mutans, Jurnal Kesehatan Gigi, Vol.1, No.1, pp.40–50.

Mardiana RN, Handayani N. (2016) Uji aktivitas antibakteri ekstrak daun sambiloto (Andrographis paniculata) terhadap Bacillus cereus dan Pseudomonas aeruginosa, Jurnal Biofarmasi, Vol.14, No.1, pp.19–24.

Putri AVAA, Hafida N, Megawati V. (2017). Pengaruh daya antibakteri ekstrak daun stevia (Stevia rebaudiana bertoni) pada konsentrasi 5%,10%, 20%, 40% dan 80% terhadap Streptococcus mutans (in vitro). Jurnal ilmu Kedokteran gigi, Vol.1, No.1, pp.9–14.

Utami LA, Putri DH. (2020). The effect of ethanol solvent concentration on antimicrobial activities the extract of andalas endophytic bacteria (Morus macroura miq) fermentation product, Jurnal Eksata Berkala Ilmiah Bidang MIPA, Vol.21, No.01, pp.1–6.

Eolia C, Syahputra A. (2019). Efektivitas antibakteri ekstrak etanol daun tin (Ficus carica Linn.) terhadap bakteri Porphyromonas gingivalis secara in vitro, Jurnal Kedokteran Gigi Univesitas Padjadjaran, Vol.31, No.3, pp.171–177.

Luginda RA, Sari BL, Indriani L. (2018). Pengaruh variasi konsentrasi pelarut etanol terhadap kadar flavonoid total daun beluntas (Pluchea indica Less) dengan metode Microwave–Assisted Extraction (MAE), Jurnal Bidang Farmasi, No.1, Vol.1, pp.1-9

Nahak MM, Tedjasulaksana R, Sumerti NN. (2017). Ability difference of beluntas leaf (Pluchea indica L) ethanol extract and avocado leaf (Persea americana Mill) ethanol extract in Inhibiting caries-causing Streptococcus mutans bacteria Growth, Bali Medical Journal, Vol.6, No.3, pp.387–390.

Agustin BA, Puspawaty N, Rizal Maarif R. (2018). Aktivitas Antibakteri Kombinasi Ekstrak Etanolik Daun Beluntas (Pluchaea indica Less.) dan Meniran (Phyllanthus niruri L .), Jurnal Biomedika, Vol.12, No.2, pp.80–87.

Alibasyah ZM, Andayani R, Farhana A. (2016). Potensi antibakteri ekstrak jahe (Zingiber officinale roscoe) terhadap Porphyromonas gingivalis. Jurnal of Syiah Kuala Dentistry Soeciety, Vol.1, No.2, pp.147–152.

Widyawati PS, Wijaya H, Hadrjosworo PS, Sajuthi D. (2011). Evaluasi aktivitas antioksidatif ekstrak daun beluntas (Pluchea indica) berdasarkan perbedaan ruas daun, Jurnal Teknolologi Pangan, Vol.5, No.1, pp.1–14.

Marion JJC, Duque TM. (2012). Efficiency of different concentrations of sodium hypochlorite during endodontic treatment. Dental Press Endododontics, Vol.2, No.4, pp.32–37.

Torabinejad M. Root canal irrigants and disinfectant. In: American Association of Endodontics. 2011.


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