Antibiofilm Ekstrak Etanol Biji Alpukat (Persea Americana) Terhadap Streptococcus Mutans (In Vitro)

Main Article Content

Adzra Fadla
Selviana Wulansari


Background: The most common oral disease in Indonesia is caries. Streptococcus mutans is the main cause of caries bacteria and has the ability to communicate with each other in biofilms. Avocado seeds (Persea Americana) contain flavonoids, saponins, alkanoids, terpenoids, steroids and tannins which have antibacterial activity. Objective: to determine the effect of ethanol extract of avocado seeds on S.mutans biofilm growth. Methods: the effect of ethanol extract of avocado seeds (P.americana) on the growth of S.mutans ATCC 25175 biofilm was evaluated by the crystal violet staining method carried out at the Micore Laboratory of Trisakti University. S.mutans bacteria were cultured using Brain Heart Infussion Broth (BHI-B) AT 37°C for 24 hours anaerobically. The ethanol extract of avocado seeds (P.americana) was diluted into 5 concentrations: 100%, 50%, 25%, 12.5%, and 6.25% with aquades. CHX (0.2%) became positive control and untreated biofilm became negative control. The extract was added to the well with a biofilm and incubated for 24 hours. The mass of the biofilm was measured by 0.5% crystal violet staining and the ethanol extract of avocado seeds (P.americana) from each concentration group and incubation period reduced the growth of S.mutans biofilm when compared with negative controls (p<0.05) . the best concentration to reduce biofilm adhesion was 12.5% with and incubation period of 24 hours. Conclusion: Avocado seeds (P.americana) ethanol ectract can inhibit the growth of S.mutans biofilm. More research is needed on other oral bacteria.



Download data is not yet available.

Plum Analytics

Article Details

Author Biographies

Adzra Fadla

Mahasiswa, Fakultas Kedokteran Gigi, Universitas Trisakti

Selviana Wulansari

Departemen Konservasi Gigi, Fakultas Kedokteran Gigi, Universitas Trisakti


Yadav khushbu, Prakash satyam. Dental caries: A review. Asian J Biomed Pharm Sci. 2016.

Kementerian Kesehatan RI. 2018. Riset Kesehatan Dasar. Jakarta: Kementerian Kesehatan RI.

Quock RL. Dental caries: A current understanding and implications. J Nat Sci JNSCI. 2015 Jan 1;1(1):27.

Forssten SD, Björklund M, Ouwehand AC. Streptococcus mutans, caries and simulation models. Nutrients. 2010/03/02 ed. 2010 Mar;2(3):290–8.

Almohefer SA, Levon JA, Gregory RL, Eckert GJ, Lippert F. Caries lesion remineralization with fluoride toothpastes and chlorhexidine - effects of application timing and toothpaste surfactant. J Appl Oral Sci. 2018;26.

Fiorillo L. Chlorhexidine Gel Use in the Oral District: A Systematic Review. Gels Basel Switz. 2019 Jun 11;5(2):31.

Widyarman AS, Lay S, Wendhita I. Indonesian mangosteen fruit (Garcinia mangostana L.) peel extract inhibits Streptococcus mutans and Porphyromonas gingivalis in biofilms in vitro. Contemp Clin Dent 201910123‐8.

Bogino PC, de las Mercedes Oliva M, Sorroche FG, Giordano W. The role of bacterial biofilms and surfacecomponents in plant-bacterial associations. Int J Mol Sci.2013 Jul 30;14(8):15838–59.

Widyarman AS, Theodorea CF. Effect of reuterin on dual-species biofilm in vitro of Streptococcus mutans and Veillonella parvula. Exp Artic J Int Dent Med Res 2019 121 77-83. 2019;12.

Hurtado-Fernández E, Fernández-Gutiérrez A, Carrasco-Pancorbo A. Avocado fruit—Persea americana. In: Rodrigues S, de Oliveira Silva E, de Brito ES, editors. Exotic Fruits. Academic Press; 2018. p. 37–48.

Oliveira JR, Oliveira FE, Higa KC. Persea americana glycolic extract: In vitro study of antimicrobial activity against Candida albicans biofilm and cytotoxicity evaluation. 2015.

Dennis, Cut Nurliza, Wulandari Savitri. Antibacterial effect of ethanol extract of the avocado seed (Persea Americana Mill.) as an alternative root canal Irrigants against Porphyromonas Gingivalis (In Vitro). Int J Appl Dent Sci 2017 31 89-93.

Nahar CL. Antibacterial effectiveness of avocado seed (Persea americana Mill.) extract on Streptococcus mutans. Vol. 7 No. 1 (2018): Vol 7 No 1 April 2018.

Bujung AH, Homenta H, Khoman JA. Uji daya hambat ekstrak biji buah alpukat (Persea americana Mill.) terhadap pertumbuhan Streptococcus mutans. E-GiGi. 2017 Jul 7;5(2).

Sánchez E, Rivas Morales C, Castillo S, Leos-Rivas C, García-Becerra L, Ortiz Martínez DM. Antibacterial and Antibiofilm Activity of Methanolic Plant Extracts against Nosocomial Microorganisms. Selli S, editor. Evid Based Complement Alternat Med. 2016 Jun 27;2016:1572697.

Sieniawska E. Activities of Tannins – from in Vitro Studies to Clinical Trials. Nat Prod Commun. 2015 Nov 1;10(11):1934578X1501001118.

Desai S, Desai DG, Kaur H. Saponins and their biological activities. Pharma Times. 2009 Mar 1;41:13–6.

Mathur S, Mathur T, Srivastava R, Khatri R. Chlorhexidine: The Gold Standard in Chemical Plaque Control. Natl J Physiol Pharm Pharmacol. 2011 Jan 1;1.

Alford JA, Pierce DA. Production of lipase by Pseudomonas fragi in a synthetic medium. J Bacteriol. 1963 Jul 1;86(1):24.